Journal:

Article Title: Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from Fc?RII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression

doi: 10.1128/JVI.77.21.11332-11346.2003

Figure Lengend Snippet: MV-N binding on different cell types

Article Snippet: The cell lines 3D1 (murine cortical TEC), HeLa (epithelial human carcinoma from cervix), MeWo (fibroblastic human melanoma from lymph node), NIH 3T3 (mouse fibroblasts from embryo), Jurkat (human acute T-cell leukemia), and Vero (monkey fibroblast from kidney) were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques: Binding Assay, Inhibition, Expressing

Journal: Clinical and Developmental Immunology

Article Title: Modulatory Effect of 1,25-Dihydroxyvitamin D 3 on IL1 β -Induced RANKL, OPG, TNF α , and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A

doi: 10.1155/2013/160123

Figure Lengend Snippet: Effect of 1,25(OH) 2 D 3 on IL β -induced TNF α and IL-6 production in MH7A. MH7A cells were stimulated with 20 ng/mL IL1 β and then treated with different concentrations of 1,25(OH) 2 D 3 (0.1 nM, 1 nM, 10 nM, and 100 nM) for 48 h. The effects of 1,25(OH) 2 D 3 on IL β -induced TNF α (a) and IL-6 (b) mRNA expression in MH7A was analyzed by real-time PCR, and the production of IL-6 in supernatants was shown in (c). The data shown are the mean ± SD for three independent experiments, each in triplicate. * P < 0.05 compared to cells cultured with IL-1 β alone. ** P < 0.01 compared to cells cultured with IL-1 β alone.

Article Snippet: Cytokine production was determined in cell culture supernatants using ELISA specific for human IL-6 and TNF α (BOSTER-BIO; Wuhan; China) following manufacturer's guidelines.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

Journal: Frontiers in Pharmacology

Article Title: Lymph Nodes-On-Chip: Promising Immune Platforms for Pharmacological and Toxicological Applications

doi: 10.3389/fphar.2021.711307

Figure Lengend Snippet: In vitro engineering of human Lymph node-on-a-Chip. (A) Schematic of the human native lymph node. (B) Requirements for in vitro biomimetic lymph node-on-chip. Requirements include: Biomimetic scaffold on which cells can be seeded or grown, cellular components (immune cells and stromal cells), appropriate extracellular matrix components, dynamic flow conditions, key features of the native lymph node including compartmentalization.

Article Snippet: An attempt to model the human lymph node is a commercial perfusion bioreactor system named the Human Artificial Lymph Node reactor (HuALN ® , ProBioGen AG, Berlin, Germany) or HIRIS TM III bioreactor ( ) .

Techniques: In Vitro

Journal: Frontiers in Pharmacology

Article Title: Lymph Nodes-On-Chip: Promising Immune Platforms for Pharmacological and Toxicological Applications

doi: 10.3389/fphar.2021.711307

Figure Lengend Snippet: Summary of existing attempts to develop in vitro lymph nodes on-chip.

Article Snippet: An attempt to model the human lymph node is a commercial perfusion bioreactor system named the Human Artificial Lymph Node reactor (HuALN ® , ProBioGen AG, Berlin, Germany) or HIRIS TM III bioreactor ( ) .

Techniques: In Vitro, Scaffolding, Chemotaxis Assay, Diffusion-based Assay, Derivative Assay, Migration, Adhesive, Blocking Assay, Expressing, Activation Assay, Shear, Purification, Vaccines, Immunopeptidomics